FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109082
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.042.722678
TCGCTCT10000.038.1576774
GTCTTGC19950.037.91671
CTGTCGC10950.036.162189
GTCTCGC9600.036.0208661
CGCTCTG11450.031.4391335
GTATTAG7400.031.153181
TACCCGT1851.7489583E-731.133245
TCTCGCT11250.030.7378042
CTCGCTC11500.030.69243
GGTATAC5450.027.75921
GTATAAC4950.027.652381
TAATACT7050.027.5727884
TTGCTCT27750.027.2415854
GTCTCAC25400.026.0938551
CTTGCTC26300.025.197923
TAACACT10650.025.0125624
CGTTTCG1751.317351E-424.684219
TTCACCG7350.023.508775
GTATAAT7200.023.013331
Produced by FastQC (version 0.11.5)