FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260489
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8550.033.7017171
GTACTAG5300.032.620721
TCTGTCG8300.031.2278698
CCGTTCG1204.22844E-429.9989979
CTGTCGC9700.028.9474859
CGGCGAA1000.006286007428.7990347
GTCTTGC19400.028.2208461
TCGCTCT10000.027.3590834
GTGTAAC6000.025.2130991
GTATTAA7750.024.1673951
GTACTGA7250.023.8468721
TCTCGCT11000.023.5758842
CTTACGC2151.9625433E-523.4530073
GTGTAAA13550.023.3922251
GTCCTAT6050.022.6233231
CGCTCTG11100.022.0533165
GTATTAC5151.8189894E-1220.9817751
CTCGCTC11700.020.9330273
GTCTCAC25900.020.582121
GCTTACG2455.3762058E-520.582122
Produced by FastQC (version 0.11.5)