FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954356
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.032.962898
GTCTCGC10050.030.1056161
TCGCTCT10800.029.999124
CTGTCGC9750.029.5375969
GTGTTAC8150.028.285031
GTATTAG7150.028.2108551
CTCGCTC10900.027.0942523
GTCGTAA1100.00997964626.1957971
TCTCGCT11650.025.970942
TACCCCG2351.4145971E-624.509925
GTACGGA1500.001551417524.0128141
CGCTCTG12200.023.6058655
GTGTACG2202.3430859E-522.9213221
GTCTTGC20050.022.6355171
CGCTCTA1600.00226555922.499345
GTATTAA6100.022.4382021
GTGTTAG14250.021.7379131
GTGTAAG9950.021.720131
TACACCG1700.003221076721.175855
GTATTAT7500.021.1312751
Produced by FastQC (version 0.11.5)