FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954356
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA800.002106599536.003031
GTCTCGC7650.032.9439471
TCTGTCG7750.032.5171978
TCGCTCT8150.030.9188884
GTATTAG6350.027.2148861
CTCGCTC8750.027.1530953
TCTCGCT9400.026.8073352
GTATAGG6000.026.402221
GTATTAT7400.026.272481
GGTATAC4400.026.1840171
GTGTTAG14150.025.4438341
CTGTCGC10300.025.1658769
GTCTTGC18150.024.2003821
GTTAGCC12250.023.5091723
GTCTTAC8400.023.1448021
GTATTAA6400.022.5018921
GTCTCAC22750.022.472221
GTATAAT6500.022.1557081
GTATAAC3656.159098E-921.7004551
TGTTAGC13950.021.6764682
Produced by FastQC (version 0.11.5)