FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124022
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7800.047.0757758
GTCTTGC19300.034.719611
CTGTCGC10800.034.6658259
TCGCTCT9750.033.9684034
GTCTCGC9200.032.893541
CGCTCTG11200.029.5707075
CTCGCTC11950.028.3326423
TCTCGCT11750.027.5945842
GGTATAC5000.027.379961
GTGTTAC9900.026.9287221
GTATTAC5350.025.5887471
GTGTAGG12400.025.5670241
GTGTAAG12150.025.500071
GTATAAA9100.023.7535781
GTCTAGA11150.023.2635961
TAATACT6850.023.1235264
GTGTAAT8450.023.02271
GTGTAAA13850.022.3700981
TTGCTCT29050.022.3058244
GTTTCAC36050.021.9808523
Produced by FastQC (version 0.11.5)