FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124022
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.037.3616688
GTCTCGC9100.037.1933251
TCGCTCT10850.031.1885264
TCTCGCT11450.029.556282
GTCTTGC19550.029.4680771
CTGTCGC11200.027.642499
GTACGGA1856.1334795E-627.2480141
GTATAGA6500.026.5892721
CTCGCTC12250.026.4486273
GTGTAAC5800.026.0735321
CGCTCTG11900.026.016465
CTATACT5750.025.0431484
GCGTAAA1500.00155470524.0042021
CTATCCG1500.001556410823.9996819
GTATTGG6950.023.831511
GGAGTGC50850.023.079345
GTCCTAC7500.023.0440331
GTTAGCC10650.022.9856113
GTATTAT6900.022.9605431
GTACTAC3803.965397E-1022.7408261
Produced by FastQC (version 0.11.5)