FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121375
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.043.0376938
CGTTTCG1105.5285527E-639.267459
CTGTCGC11200.034.066859
GGTATCG1301.7375414E-533.2263075
TCGCTCT9900.030.5413484
GTCTCGC9350.029.8103771
GTATCGA1453.6644808E-529.78916
GTATACG1306.058385E-428.211241
GTCTTGC19700.026.8078381
TAATACG1400.001041843125.7108294
GGTATAC4600.025.5127721
GTATAGC5550.025.1105441
GTCTCAC25850.024.6861971
ATCGAAC2051.3636987E-524.582068
CTCGCTC11150.024.534243
GTATTAG8750.024.3100261
CGCTCTG10150.024.1149855
GTGTTAC10050.024.0848181
GTATTAA7450.023.629281
GTTTCAC32300.022.9561253
Produced by FastQC (version 0.11.5)