FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.035.996228
GTCTTGC22650.032.8700681
CTGTCGC12750.029.9262689
GTCTCGC10400.029.8859231
GTATTAT7800.027.800861
TTATACT9300.027.0939274
TTCACCG7200.026.9971645
TATACGG1358.424871E-426.6638685
TCGCTCT12200.026.5545884
GTCTTAC11600.026.1711541
GTATAAT8500.025.5113751
TAATACT7350.025.4667134
GTTTCAC39650.025.0976683
TCTCGCT13250.025.045272
GTATAGA8450.024.806921
ACCGTGT8450.024.7074668
GGGTTTC44150.024.394231
GTGTATA15350.024.0155961
CGCTCTG12000.023.9974785
TAAACCG1550.001884726823.2233685
Produced by FastQC (version 0.11.5)