FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.040.3432668
GTCTTGC22350.036.4134981
CTGTCGC13000.035.442159
GTCTCGC11150.033.536081
TCGCTCT12350.032.644724
CGCGTCG950.004893687630.3129545
GTGTTAG18450.029.0097141
GTATTAA7450.028.540311
GTATTAT8450.027.7658041
GTCTAAT8300.026.5008721
CGCTCTG13250.026.080585
CTCGCTC13900.025.8963553
TCTCGCT14500.024.8877582
GTATACG1801.4095429E-424.4396921
TTGCTCT32300.024.294944
GTGTTAC13350.024.1650891
GTGTAAC6800.022.6426561
GTCTTAC10050.022.6158331
GTATAAA12050.022.5129091
GTGTAAA16000.021.9957221
Produced by FastQC (version 0.11.5)