FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358674
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.037.8069768
GTCTCGC9250.032.8067631
CTGTCGC11400.030.9448479
GGTATAC5500.030.2148861
TCGCTCT10150.029.7906784
GTCTTGC22700.029.6014441
GTATAAT6850.028.4792791
TCTCGCT11350.026.052532
CTCGCTC11750.025.7695853
GTATTAG8050.025.1314751
GTGTTAG15300.024.5565721
GTATAGG6200.024.4727881
TAATACT6250.024.1900314
GTATTGG7000.023.7402671
TTATACT7050.023.487454
GTATAGA6550.023.1650811
GTATTAA7850.023.0105061
TCGATCT8450.023.0040449
CTGTTGC24150.022.9546539
GAACCGT2852.9764487E-722.734996
Produced by FastQC (version 0.11.5)