FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358674
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.037.9273768
GTCTTGC19750.034.172951
GTCTCGC10200.033.0841061
CTGTCGC12000.032.3962979
TCGCTCT12450.030.0694894
GTACTAG4650.028.3975771
TCTCGCT12850.026.9650422
CTCGCTC12650.026.7473663
TGTACCG1907.561728E-626.5238485
CGCTCTG14150.026.4569025
GTATTAG8250.025.7872941
GTATAGC4850.024.2013721
GTGTTAG14900.024.1252351
GTCTAAT7050.023.9331821
GTCTTAC10900.023.5560971
GTATTGT7550.023.3198641
TATTCCG1550.0018845523.2236465
GTGCGAC1902.0258586E-423.1664451
GTACTAT6100.022.8499641
GGTATAC5550.022.4707571
Produced by FastQC (version 0.11.5)