FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243407
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9250.036.4417531
GTCTTGC20850.034.0917171
TCTGTCG9000.031.197448
TCGCTCT10500.029.4832944
GTATAGG6100.028.8313261
CTGTCGC11550.028.0496489
TAATACT7000.027.7691524
GTATTAA7200.027.4798561
GTATAAC4650.026.7903961
GTATTAC6300.026.7528741
GTCTTAC8700.026.1111261
TCTCGCT12600.025.2082232
GTGTTAG16950.025.0750311
GCGTAGT2701.4886427E-724.4265381
GTACAGA13250.024.3343641
CGTACAC2701.7666935E-723.9974963
GTACTAT6200.023.6385841
CTCGCTC13150.023.541273
GTGTTAC10300.023.4779361
GTTTCAC30650.023.0188063
Produced by FastQC (version 0.11.5)