FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160622
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.044.7808761
TCGCTCT10100.042.7687074
TCTGTCG9300.037.932328
TCTCGCT12400.034.9109462
CTCGCTC12200.034.2242773
CTGTCGC10650.033.89
CGCTCTG11700.033.2279975
GTCTTGC22200.032.3623471
GTACGTC1255.3736946E-428.7955993
GTATTAA6800.028.0305331
TATACTG6500.026.5823975
GTACGGC1450.001149885625.2795281
GTGTTAC9000.025.2514361
TTGCTCT27550.024.8255164
ATGGCGT9150.024.3919736
GTACGGT2151.713082E-523.8685781
GCGTGAA8650.023.3044159
TCTGTCA29050.023.295828
GGGTTTC33750.023.2421841
GTGTTAG15500.023.175621
Produced by FastQC (version 0.11.5)