FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213556
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.049.8115548
CTGTCGC10750.037.5034689
GTCTCGC10050.032.3673861
TCGCTCT10550.030.7080234
GTCTTGC21900.030.3672141
TAATACT7950.028.9784184
GTATAAT6350.027.3211331
TCTCGCT12150.026.72032
CTCGCTC11950.026.5553653
CGCTCTG11600.025.4458945
ACCGTGT7650.025.4093848
GGTATAC4650.024.8730051
GGGTTTC37650.024.383721
GTATATA11200.023.8805831
GTTTCAC35800.023.3691253
TTGCTCT31300.023.2310524
GTTAGCC12250.022.9612753
GTGTTAG15450.022.4581491
GTGTAAA15450.022.4581491
TAACCCG2252.8011442E-522.3979025
Produced by FastQC (version 0.11.5)