FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425812
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10350.038.2548941
TCTGTCG9950.036.9018678
GTCTTGC21100.034.7497061
TCGCTCT12950.031.6894954
CTGTCGC13300.030.313619
GTACGAT1702.7605347E-630.1913621
ATACCGT2151.9395884E-830.1386136
CTCGCTC13250.029.8834153
GTGTTAC10750.029.3287491
TCTCGCT13400.029.0877912
TATACCG1504.6155474E-528.7985235
GTATTAG8050.027.3249241
CGCTCTG14650.026.537895
GGTATAC5550.025.1011811
GCGTAGT2401.4501238E-624.4406261
ATACCGA1500.001556631823.9992646
TTGCTCT31250.023.9603714
GTGTAAT11200.022.9130861
GTATTAA7100.022.7194561
CTTGCTC32000.022.4974563
Produced by FastQC (version 0.11.5)