FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331629
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.033.2833828
GTATAAT7450.031.49961
GTCTTGC21300.029.9537331
GTCTCGC10100.029.0435661
TCGCTCT11250.028.7973614
GTGTAAA15400.026.1910721
AACGGCG1400.00104161425.7119315
GTGTAAT11050.025.2192781
CTGTCGC13050.024.8253129
GTATTAT7800.024.4450021
TTAACGC1801.5961565E-423.9957433
GTGTTAC10850.023.6564521
GCGTAAT1550.001692578723.6564521
GTGTATA15400.022.3814621
GTATTGC6950.022.1587771
GTCTAAA9600.022.1532821
ACCGTGT7500.022.0779768
GTACATA8000.022.0005021
GTGTACG3004.0842133E-722.00051
GGGTTTC38850.021.896681
Produced by FastQC (version 0.11.5)