FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100102
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.045.7763528
CTGTCGC10750.037.5037049
GTCTCGC9950.034.1542661
GTCTTGC18800.031.5374181
TCGCTCT10200.031.0561144
TCTCGCT11700.028.3687112
CGCTCTG11350.027.9094585
GTATTAC6000.026.5119441
CTCGCTC11850.025.5599653
GTACCGT1450.001246274124.9328631
GTATTAA7350.024.593641
GTACTAG4550.023.8369141
GGTATAC4251.8189894E-1223.8182161
GTGTAAT9150.022.9164331
GTATAGA6650.022.8332541
GTATAAG6350.022.7733231
GTATAAT6450.022.4202481
GTGTATA11300.022.3954471
GTACTAC4659.094947E-1221.7693371
GGGTAAC8350.021.6482961
Produced by FastQC (version 0.11.5)