FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405609
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10700.040.4023061
TCGCTCT11750.034.926864
TCTGTCG10400.034.6145978
TCTCGCT12800.031.5025562
GTATAAT5550.031.1570911
GTGTACG1902.1950109E-730.3371681
CGCTCTG12950.030.022485
CTGTCGC13350.029.6622459
GTCTTGC19600.029.0408741
TACCCGT2303.7356585E-828.1732715
GTATTAG7750.027.8906211
CTCGCTC13750.027.7549783
GACGTAC1050.00794799627.4479121
TAGGTCG1050.007976267527.4279445
GTGTAAT9500.027.303451
GGGTTTC39000.024.3864151
GTACTAC3851.8189894E-1124.328831
GTATTAC5050.024.2547151
GTGTAAC6600.024.0169241
GTATACG1500.00154997624.0169221
Produced by FastQC (version 0.11.5)