FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405609
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG903.0013325E-1063.9969189
TCTGTCG9450.041.1408778
GTCTCGC10200.039.70331
TACCGAT750.001535488538.398157
GTCTTGC20300.036.3364831
CTGTCGC12650.035.2868429
TCGCTCT11300.034.4054264
TCTCGCT12450.030.6491282
GTGTTAG13950.029.5487671
CTCGCTC13550.028.1610093
TTGCTCT26900.028.102744
ATACCGA1306.7553E-427.6909756
GTACAGA12200.026.0814421
GTACTAT4450.026.0015181
CTTGCTC27850.025.334513
GTACTAG4001.8189894E-1225.3108541
GTGTTAC9050.024.7714751
GTATAAC4151.8189894E-1224.3960021
CGCTCTG15950.024.3750045
GTATAAT5150.023.871541
Produced by FastQC (version 0.11.5)