FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427827
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7900.041.0120168
GTCTCGC9350.033.1390841
TCGCTCT10850.032.515624
CTGTCGC9950.031.1150939
GTCTTGC24000.030.324511
CGCTCTG11500.028.1734715
TCTCGCT11800.027.4600282
GTATAAC4050.026.6882381
GTATTAT7450.026.1150671
GTCGCGC1400.001036170425.7350851
CTCGCTC13300.025.4458333
GTGTAAG14750.025.4035841
GTATTAG7700.025.2671741
GCGTTAC1450.001271445324.8476681
GTCTCAC28900.023.6869661
GTATAGT6550.023.102641
GTACTAT5950.023.0101951
GTATATA9800.022.0586451
GTATAGG5900.021.983871
TTGCTCT30150.021.9698074
Produced by FastQC (version 0.11.5)