FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159206
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.035.0250558
GTCTCGC9250.033.5959781
GTCTTGC21350.030.8037831
CTGTCGC10100.030.6517379
TCGCTCT10200.029.6453914
GTATTAC5150.028.0661811
CTCGCTC11250.026.8784873
CGCTCTG11000.026.8348525
GGTATAC4850.026.822011
CCTATCG1358.42204E-426.6651659
GTATTAG7950.026.3627931
GTTTCAC31350.025.9506913
GTGTTAC9200.025.9230821
TCCGTAC1400.001041374425.712843
TATACTG7650.024.469215
TCTCGCT12700.023.8102342
GTGTAAC7000.023.7459951
GTATAAC4650.023.3130361
GTCTAGC9200.022.780891
GTGTTAG16200.022.7517971
Produced by FastQC (version 0.11.5)