FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327382
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8050.033.986878
GTCTCGC9950.032.585241
CTTGTCG2351.3624231E-930.6376639
CTGTCGC9900.029.8175649
TCGCTCT10500.029.4851024
GTCTTGC22050.027.7742161
CGCTCTG11250.027.5194285
CTCGCTC11100.027.2444363
GTATAGA7550.026.7203791
GCGCGAT5150.026.5625559
GTACGTA1100.00997250526.199851
GTATTAG9450.025.9226041
TCTCGCT12350.025.6529432
GTTTCAC35500.025.5560463
GGGTTTC40400.024.789341
TTATACT7200.023.9995024
GAACCGT2752.1075721E-723.5631486
GGGTACG1851.9106979E-423.3674341
GTATAAC5250.023.3303431
GGCTAGA14550.022.7785631
Produced by FastQC (version 0.11.5)