FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327382
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.041.5039338
GTCTCGC9950.035.589431
GTCTTGC20700.032.8175051
CTGTCGC12500.029.9505949
TCGCTCT11500.029.4247044
CGCTCTG13500.027.732035
TTCACCG8100.027.5542585
GTGTAAG11650.026.674151
GTATTAA7400.026.3681771
GTGTTAT10400.025.710851
TCTCGCT12950.025.5740872
GTATTAT7550.024.887111
CTCGCTC13550.024.4416563
GGATAAT10700.024.314581
CCGTGTT8650.024.1375949
ACCGTGT8400.023.9988738
GTCTCAC26950.023.865981
GTATAAG5500.023.6514551
TACACTA6150.023.4135325
GGGTTTC38200.023.2696481
Produced by FastQC (version 0.11.5)