FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162764
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.047.9993138
CTGTCGC11150.041.3267639
GTCTTGC20350.040.012151
GTCTCGC10300.032.88051
GTATTAG8700.032.301541
TCGCTCT11100.031.7833294
GTCTCAC27800.029.5486681
GTCTTAC9850.029.261841
GTATTGC7150.027.210441
TTAGCGC1607.15408E-527.0039843
TTGCTCT30550.026.8670444
CTTGCTC28150.025.8368473
TCTCGCT12700.024.9485632
CTCGCTC12800.024.7536533
TCTTGCT32300.024.3007982
CGCTCTG13150.024.090915
GTACTAA6650.023.8384971
GTTTCAC35050.023.0105293
CTGTTGC23500.022.6720169
GCGTTAC1600.00225480822.5178991
Produced by FastQC (version 0.11.5)