FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162764
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC907.6070195E-540.1677131
TCTGTCG9750.039.136288
GTCTCGC9200.036.9368321
TCGCTCT11250.033.9188964
TCCGATC1102.53895E-432.725458
CTGTCGC11800.032.337189
GTCTTGC18850.029.918021
GTCTTAC8400.028.4043141
GTATTAG8050.027.842961
TCTCGCT12400.026.7088072
CTACGCT1959.246367E-625.845314
GCGTAAC1400.001015926525.8221021
GTTTCAC34350.025.7808193
CTCGCTC12650.025.6118133
CGCTCTG13500.025.065855
GGGTTTC36500.023.968571
TTACACT12050.023.8996354
GTCTCAC27850.023.6246741
GTATAGG5900.023.2836591
GTGTAAT8700.023.2695711
Produced by FastQC (version 0.11.5)