FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125054
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9700.039.3646931
TCGCTCT10600.036.678764
TCTGTCG8200.035.1214878
TCTCGCT11350.032.987122
GTCTTGC20100.032.6173061
CGCTCTG11400.029.0522485
CTGTCGC9850.028.5072369
ACCGTGT7850.027.515568
CTCGCTC12900.026.7909743
GTATTAT7600.026.542831
GTACTAT5400.025.3491041
GTATAGA6300.025.1585061
CCGTGTT8450.024.7097329
TAGTACT4750.024.2523124
TTCACCG7500.023.9996815
GTTTCAC34000.023.9296573
GTGTTAC9950.023.8942621
ATAGTAC4950.023.2729683
GTTCGGA1550.001876665323.2402611
GGGTAAA8750.023.0543421
Produced by FastQC (version 0.11.5)