FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125054
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.040.5250858
GTCTCGC9350.036.337051
CTGTCGC11000.030.7622229
TCGCTCT10200.030.3515454
ATACCGG1000.00628630528.7986766
GTCTTGC21050.028.5029261
GTATTAC6150.027.0343691
GTGCTAT9050.026.3589921
GTATTGA7700.026.2863791
CTCGCTC11300.025.4855563
TCTCGCT11700.025.2307992
GCGTATA1450.001248064524.9267391
GTGTTAG14350.024.1798061
GTATAAT7100.023.4170911
GTACTAG4950.023.3656711
CGCTCTG11100.023.3502795
GTATTAG8050.023.347531
GTGTTAC9600.022.5898571
CGCTAAC1600.00226522322.5000252
GTGTAAT9700.022.3569721
Produced by FastQC (version 0.11.5)