FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255073
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT1352.2474167E-531.9996285
TCGCTCT10850.031.8521654
TCTGTCG7750.031.5867358
GTCTCGC10950.030.2643451
GTATTAT6700.029.031971
GTCTTGC22200.028.2327941
GGTATAC4500.027.2159791
CTCGCTC11500.026.2982643
CTGTCGC10050.025.7907479
TCTCGCT12300.025.7586572
TTACGCT2259.684081E-725.5997054
GTATTAG7100.025.3670061
GTATTAA7700.025.2615851
GTGTTAG15900.024.4671961
ACCGTGT7250.023.8342088
GTATTAC5500.023.577481
GGATAAC6550.023.097531
GGGTTTC40850.022.573841
CCGTGTT8100.022.2219669
GTTTCAC37650.022.185473
Produced by FastQC (version 0.11.5)