FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293562
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.034.6126441
CTGTCGC8500.033.8817069
TCTGTCG8800.033.5448158
TCGCTCT10850.030.5247654
GTCTTAC11250.026.8959031
GTGTTAC10200.025.4267981
CTCGATC7950.025.3580078
TCGATCT8000.025.199529
GTGTAAA15200.025.1201171
TCTCGAT7800.024.9226027
GCGTTAC1751.3115778E-424.7003211
CTCGCTC11450.024.5251543
GTCTTGC21450.024.182131
TCTCGCT12800.023.0635682
GGTATAC4700.022.992321
CGTTACC1902.2989183E-422.7378942
GGCTAGA14850.022.3162291
TGTACCG1650.002708830921.8177665
GTATAGA8600.021.7803211
TTCACCG7300.021.6982155
Produced by FastQC (version 0.11.5)