FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293562
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.035.998338
TCGCTCT11350.034.8890154
GTCTCGC10200.034.73051
CTGTCGC10400.031.8446779
TCTCGCT12650.028.4578022
CTCGCTC13650.027.9553593
CGCTCTG13100.027.4802425
GTCTTGC18150.025.0945261
GGTATAC5050.024.3373131
CTCGATC8050.024.1479518
GTGTATA13300.023.9175191
GTGTAAT10050.023.739031
CGTTTCG1550.00188402223.2247319
GTATTAA7900.022.8785251
GGCTAGT7950.022.7346341
TTACACT13750.022.5158124
GTCTCGA20300.022.3442786
TCTCGAT8400.022.2851667
TCGATCT8700.021.5162459
GTGTTAG14800.021.4934461
Produced by FastQC (version 0.11.5)