FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870545
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG400.00623845453.959584
TCTGTCG12850.036.3936048
GTCTCGC13000.034.7878271
TCGCTCT13450.031.5600014
GTCTTGC28450.031.535651
GTGTTAC12900.029.4030651
TCTCGCT15550.028.2232322
CTGTCGC16250.027.4511649
CTCGCTC15750.026.4944383
TTGCTCT38450.025.0735474
GTACTAG6650.024.131261
GTGTTAG20550.023.7816491
GTATAAC5900.023.4898661
CGCTCTG17200.023.0064245
GTTAGAC6950.022.7743053
GGGTTAC10300.022.6616611
GTACCGG2603.0426982E-622.4437581
CTTGCTC39250.022.1795643
GTATTAC7100.021.574461
GGGTTTC49000.021.436081
Produced by FastQC (version 0.11.5)