FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258140
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18000.033.6470071
GTCTTGC40450.031.9061341
TCTGTCG17300.030.3804248
GTATAAG12650.026.2184471
CTGTCGC22750.025.9507279
TCGCTCT20000.025.1991044
GTATAAC8850.024.4409261
TACACTG26500.023.6368945
GTGTTAC18800.023.3943841
TCTCGCT21900.023.3666062
GTATATA20100.023.3161561
TTGCTCT55150.022.9765174
GTATTAA12600.022.889121
GTATAGG11400.022.7686521
GTATTAT13150.022.4800761
GTATTAG14500.022.376091
GTGTAGA22950.022.3056721
GTGTTAG30200.022.2032051
GTTACCC14100.021.9724043
CTCGCTC23650.021.6299463
Produced by FastQC (version 0.11.5)