FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258140
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17050.036.3172348
GTCTCGC18550.032.6222421
GTCTTGC38900.030.9275341
CTGTCGC22550.029.0558479
TCGCTCT20150.026.4420624
GTATTAG14350.025.101321
GTCTTAC15950.023.9383181
GTATTAT12650.023.9186821
TCTCGCT24700.023.6247122
GTATAAT12250.023.5235211
TTGCTCT51450.022.9507644
CGCTCTG23300.022.5582625
CTCGCTC23800.022.3868733
GTCTCAC50450.021.4194621
GTGTAAT17700.021.1645241
GTTTCAC60650.021.1313823
GTGTTAC16500.020.957321
CTTGCTC52500.020.5717223
GGGTTTC70900.020.5250191
GTGTTAG28150.020.4734041
Produced by FastQC (version 0.11.5)