FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975806
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12350.032.624668
GTCTCGC13450.032.52191
GTCTTAC11500.030.4291841
TCGCTCT14200.029.8948174
GTCTTGC28300.029.1097811
TCTCGCT15600.029.0568262
GTATTAG10000.028.432271
GTATAAT8800.027.338721
CTCGCTC16700.026.7120673
CGCTCTG15650.024.8258655
CTGTCGC17450.024.3266079
GTATTAT10450.023.7197171
GTATTAA10350.022.5401361
GGTTACG1952.5200413E-422.431771
GCGTTAG3004.3239925E-721.8709751
GTGTATA19550.021.6285881
GTATACG2754.9716764E-621.208221
TTGCTCT38450.021.1453234
GTATTGG10700.021.1215041
GGAGTGC63750.020.8793265
Produced by FastQC (version 0.11.5)