FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975806
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13300.035.3617551
GTATTAG11200.032.8063131
TCTGTCG14650.031.4534788
TCGCTCT16750.027.5105194
GTCTTGC30000.027.4348281
CTCGCTC17600.026.5914253
GTGTAAC10150.025.3400481
CGCTCTG17000.024.98835
TCTCGCT18250.024.858622
TACCCGT2051.3624915E-524.5854325
GTGTTAG21500.024.2675151
GGTATAC6200.023.7052061
TACACTG20200.023.1683755
CTGTCGC21500.022.436899
GTATAAC6000.022.0458411
GTTTCAC45350.021.592483
TTCACCG8350.021.5569425
GTATTAA8950.021.3479291
GTATAAG9650.021.3224031
GTGTTAC11450.021.1794131
Produced by FastQC (version 0.11.5)