FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397115
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.040.371158
GTCTTGC22350.032.5434651
GTCTCGC10450.032.017171
TCGCTCT11600.028.5326124
TCTCGCT12150.025.4644262
TTGCTCT31350.025.246254
GTCGTAG2051.2593022E-524.8362221
GTGTAAT10250.024.8362221
CTGTCGC17150.024.7541319
CTCGCTC12900.024.5417023
GGTATAC6000.024.2448841
GTTAGCC12200.024.1805253
GTGTTAG15950.023.7128641
GTCTCAC27150.023.5751361
GTATAAG6800.023.53181
TAGTACT6150.023.3989624
CGCTCTG14100.022.9633435
GTATTAG8000.022.7295781
CTAGTAC5700.022.7216223
GTTTCAC36950.022.5883883
Produced by FastQC (version 0.11.5)