FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719579
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10950.043.6942561
TCTGTCG11600.042.8296938
TCGCTCT13300.041.144884
TCTCGCT15550.031.0297412
CGCTCTG15700.030.7276275
CTCGCTC15650.030.3673883
GTCTTAC11550.029.9530431
GTCTTGC24100.029.6264611
GTATAAC4750.029.4432071
CTGTCGC17950.028.4805099
GGTATAC5800.027.920281
GTATTAA6850.026.8642371
TAATACT7800.026.7705464
GTATTAG9300.026.118971
GTATAAG8350.023.8013941
TTGCTCT33100.023.0585354
GCGTTAC1600.001992446123.0025021
GTCTCAC32450.022.0030121
GTTTCAC39850.021.8642653
GTACTAT6100.021.7203981
Produced by FastQC (version 0.11.5)