FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018465
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13750.036.1319128
GTCTCGC13100.035.4013181
GTCTTGC29150.034.59651
TCGCTCT14650.033.4196134
GTATTAC6100.030.1689591
GGTATAC6700.029.6646441
GTATTAA8500.028.5788761
CGCTCTG16750.026.6510415
CTCGCTC17400.026.4826153
GTGTTAG21900.025.8819371
CTGTCGC20850.024.8639989
TTGCTCT37950.024.8536194
TCTCGCT18300.024.7900372
GTCTCAC34500.023.8973161
GCGCTAA2201.9829422E-523.4220831
CTTGCTC39500.023.1492423
GTGTTAC12200.022.9284081
GTGTAAT11000.022.752881
CGTTTCG1902.2994407E-422.7374739
TTCACCG8900.022.6519375
Produced by FastQC (version 0.11.5)