FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631558
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15450.039.1443188
GTCTCGC17850.032.318551
TCGCTCT19250.031.0431124
CTGTCGC20850.029.6968449
GTCTTGC34750.029.674361
GTATTAG13050.027.0760731
GTGTAAC10200.026.8647961
CGCTCTG21500.026.7897995
GTATATA17700.026.4813561
GTATTAA10300.025.9038681
TCTCGCT22100.025.7654272
GTATTAC8150.025.6590441
GTGTTAG26200.025.3213391
GTGTAAA21300.025.0525681
CTCGCTC21800.024.125893
GTATACG2452.006138E-623.5463731
GTGTTAC16500.022.2887821
GTCTCAC44250.021.8369331
GTATTAT12900.021.800931
GTACCGT3055.632337E-721.2785871
Produced by FastQC (version 0.11.5)