FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631558
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC36350.033.286721
TCTGTCG15750.031.0858638
GTATTAC8750.028.8088651
GGTATAC8400.025.72221
CTGTCGC20000.025.5601239
GTCTCGC16850.025.218441
GTATTAA10000.025.2077581
TTGCTCT48650.023.9754494
TCGCTCT19550.023.5704464
TTACACT17150.023.090494
CTTGCTC46700.022.663923
GTATTAT10600.022.4219931
TATTACG2252.7931106E-522.4068932
GTCTAAC8550.021.9014761
GTATAAT9900.021.8248981
TAATACT11700.021.5385674
GTATTGG11400.021.4802931
GTGTTAG23850.021.440561
GTCTTAC13150.021.3601861
GTCTCAC44550.021.3399011
Produced by FastQC (version 0.11.5)