FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681549
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16150.038.78518
GTCTCGC16750.033.5751341
GTCTTGC36800.029.9765451
CTGTCGC21250.029.4766779
TCGCTCT16950.028.8840054
TCTCGCT19850.026.5057982
GTATTAT10650.025.725981
CTCGCTC20200.025.6785623
GTATTAA12150.025.5170331
GTATTAG13400.024.75091
GTATAGA12900.024.59241
GTCTTAC16750.024.5356751
GGTATAC6650.023.852781
CGCTCTG20800.023.5376875
GTGTTAG29500.023.4631961
GTCTCAC41150.023.1282141
GTATAAT11500.022.5705761
GTGTAGA20800.022.5313911
TAATACT11450.022.0080094
TTGCTCT47800.021.8402844
Produced by FastQC (version 0.11.5)