FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3949004
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17600.039.683088
GTCTTGC36550.033.3034061
CTGTCGC23150.030.7914759
GTCTCGC17050.030.415671
GTATTAA12450.026.6120151
TCGCTCT19000.026.14824
TAATACT11200.024.4293484
GTCTCAC47700.023.555671
GTGTTAC16550.023.5009291
GTGTAAA24000.022.5081231
GTATTAG13300.022.2035031
GTCTTAC16900.022.1618461
GGGTTTC64450.022.0157051
GTATTGG15150.021.869281
GGTATAC7350.021.5588021
GTATAAG11700.021.546241
TTGCTCT53200.021.519484
GTGTAAC12150.021.3410381
GTATTAT11200.021.2219451
GTACTAG7550.020.9877091
Produced by FastQC (version 0.11.5)