FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843931
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16750.035.6763428
GTCTTGC35400.034.228381
GTCTCGC15950.031.2010151
CTGTCGC21400.028.9335469
TCGCTCT17850.028.2342854
GTGTTAC16750.027.557861
TCTCGCT19700.025.9824492
CTCGCTC19700.025.9729773
TTGCTCT49350.025.8227864
GTATAGG10300.024.5081841
GTATTAT13900.023.8684021
CGCTCTG21950.023.2884655
CTTGCTC46900.022.8951243
GTGTTAG26150.022.6163481
GTATTAG12550.022.4130611
GTCTTAC15800.022.3675961
GTGTAAA24050.022.1920261
GTTTCAC60400.021.5958983
GGGTTTC68650.021.4323581
GTGTAGA20050.021.2235451
Produced by FastQC (version 0.11.5)