FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754878
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.032.2757078
GTCTCGC12650.030.7456341
TCGCTCT15350.026.7360294
GTCTTGC23800.025.1177861
CTGTCGC15850.024.0755969
GTACTAA4550.023.7443391
GTCTAGA10750.023.4498371
GTATTAG6850.023.1319541
GTCTAAT6550.023.0918221
GTATTAA5450.022.4663561
TCTCGCT16800.021.8645762
GTCTCAC29650.021.6194931
GGGTATG9350.021.5688321
CGCTCTG17400.021.5171385
CTCGCTC16350.021.1375143
GGGTAAG12050.019.724551
GTAGCAC8050.019.6769243
GTCTTAC8800.019.6430441
GTGTAAA12200.019.4820351
GTACAGT11300.019.1215481
Produced by FastQC (version 0.11.5)