FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299358
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13950.036.643758
GTCTCGC15200.035.1034161
CTGTCGC18150.031.3376359
GTCTTGC31800.031.2905481
TCGCTCT15850.030.8888684
CGTTTCG1453.662694E-529.7919569
TCTCGCT17650.026.5423152
GTATTAC8000.026.1378141
GTTACGA1701.0767416E-425.4288983
CTCGCTC19100.024.8963553
CGCTCTG18550.024.840395
GTATTAG11350.024.140651
GTATTAA9700.023.786991
GTGTTAC14250.022.7697831
TTGCTCT48100.022.4526984
GTATTAT11000.021.6312941
GTGTAGC16700.021.5881181
GTATAAG10550.021.1870481
TAGTACT7500.021.1195074
TAATACT10650.020.9572564
Produced by FastQC (version 0.11.5)