FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366230
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14350.044.6551678
GTCTCGC14600.036.516031
CTGTCGC19150.033.0862469
TCGCTCT15650.030.8243584
GTCTTGC31150.029.1418511
GTATTAA9850.026.3312171
CTCGCTC18650.026.2520773
GCGTAAT1658.792802E-526.198231
TCTCGCT19250.026.1982292
GTCTCAC42050.024.6718161
CGCTCTG17900.023.7319055
GGTATAC6800.023.308721
GTGTAAA21050.023.2734871
CGTAATA1550.001876843623.2403642
GTGTAAC9650.022.397451
GTATTAG11950.022.3068621
GTATTAC8500.021.1897451
GTATAAG10900.021.1508641
TAATACT10600.021.0566584
TTATACT10600.021.0566584
Produced by FastQC (version 0.11.5)