FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475941
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14600.041.42588
GTCTCGC16100.036.905431
TCGCTCT18550.035.3217284
CGCTCTG19250.031.0450135
CTGTCGC19700.030.6982639
GTCTTGC33050.030.4751341
GTATTAG11050.029.508871
CTCGCTC20950.028.8695323
TAGTCCG1753.986441E-628.8007955
TCTCGCT19700.028.567172
GGATACG3108.6583896E-1025.711851
GTATTGG12350.024.0557671
GTGTAAC10350.023.8035031
GTGTTAT13150.023.694361
GTATTAC7700.023.526191
TAATACT10550.023.2044324
GTATAGA11000.023.0556661
GTATAAC6600.023.0556661
TATACGG2202.315794E-522.956952
GTCTCAC39900.022.7007081
Produced by FastQC (version 0.11.5)