FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165249
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.037.4148648
GTCTCGC13600.031.3590721
GTCTTGC30950.029.6615051
CTGTCGC17150.028.9660269
TCGCTCT15900.027.1740364
GTCGTAA1358.2278776E-426.7723431
TTATACT9450.026.6708154
TCGACCG1358.4233715E-426.6649159
GTATTAG10650.026.470681
GGATAAC9200.025.928431
GTGTTAG21650.025.7088661
GTATAAC5950.025.5124661
TCTCGCT18250.024.495042
GTGTAAA19150.023.7805481
GTCTTAC14050.023.6663681
TTCACCG9450.023.617875
CGCTCTG18500.023.3501855
TTACACT15100.022.8909764
GTGTTAC13200.022.452261
CTCGCTC18300.022.4506473
Produced by FastQC (version 0.11.5)