FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165249
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13950.042.1015971
TCTGTCG14350.035.6252338
TCTCGCT17050.033.444052
TCGCTCT16700.033.199044
CTCGCTC17400.032.6910673
GTCTTGC32900.031.2953761
CGCTCTG17450.030.5342735
CTGTCGC18950.029.2544299
GTATTAG11050.028.21591
GTGTTAC12300.025.9379311
GTGTTAG19600.024.7860031
GTTAGCC15050.024.3997253
GTCTCAC37000.024.3000831
GTCTTAC13300.023.987711
TTGCTCT43100.023.8896644
GTATAAT7900.022.9456631
GTACGGA2552.7074566E-622.7477041
GTATTAT10850.022.7214971
GGTATAC6400.022.6588441
CTTGCTC41500.022.5551913
Produced by FastQC (version 0.11.5)