FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762373
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC14800.027.4478821
GAACGAT2105.29888E-727.4299436
TCTGTCG6750.024.5345638
GTATAAG4201.8189894E-1224.1802771
GTGTTAG8900.023.6369021
TCGCTCT9000.023.2011624
GTATTAA3803.619789E-1022.9076331
GTGTAAA8900.022.8218361
GTCTTAC5800.022.5126741
GTACAGA7850.022.1780911
GTACATA3604.822141E-922.1652571
GTATAAA5600.022.0213261
GTCTCGC8250.021.982071
GTATTAG4409.640644E-1121.432521
CGCTCTG10150.021.2818555
TTAGGAC5101.8189894E-1221.1775323
GTCCTAC5151.8189894E-1221.12841
GTAGAAC4451.2551027E-1021.0347613
GTGTTAC5251.8189894E-1220.7259521
GTGTAAG7800.020.4602381
Produced by FastQC (version 0.11.5)